Zinselmeyer B H, Lynch J N, Zhang X, Aoshi T, Miller M J
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Inflamm Res. 2008 Mar;57(3):93-6. doi: 10.1007/s00011-007-7195-y.
Leukocyte recruitment is a key host defense mechanism to infection and a salient feature of autoimmune diseases such as arthritis. The cell dynamics of these processes are difficult to study due to the challenge of tracking cells flowing in the circulation and migrating through light scattering tissues. Here, we describe a noninvasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. In the absence of inflammation, cells moved > several hundred microm/s in vessels and only rarely adhered to endothelium or entered the tissue parenchyma. In response to bacterial infection, neutrophils moved in small capillaries at reduced speeds of (14-45 microm/min) and rolled in larger vessels at 5-60 microm/min. Within minutes of adoptive transfer, neutrophils entered the connective tissue and crawled with a median velocity of 7.3 microm/min. 2P imaging has excellent spatiotemporal resolution and is a promising in vivo approach to study the cellular basis of inflammation.
白细胞募集是机体抵御感染的关键防御机制，也是关节炎等自身免疫性疾病的一个显著特征。由于追踪循环中流动并通过光散射组织迁移的细胞具有挑战性，这些过程的细胞动力学难以研究。在此，我们描述一种非侵入性双光子（2P）显微镜方法，用于研究小鼠足垫中的白细胞归巢。在没有炎症的情况下，细胞在血管中以大于数百微米/秒的速度移动，很少黏附于内皮或进入组织实质。响应细菌感染时，中性粒细胞在小毛细血管中以较低速度（14 - 45微米/分钟）移动，在较大血管中以5 - 60微米/分钟的速度滚动。在过继转移几分钟内，中性粒细胞进入结缔组织并以7.3微米/分钟的中位速度爬行。双光子成像具有出色的时空分辨率，是研究炎症细胞基础的一种有前景的体内方法。
