Chen Qian, Zhao Zhong-Zhong, Peng Rong, Wang Ying-Yu, Xie Zhao, Wang Ze-Zhou, Li Jiang-Lin, Lv Xue-Bing, Wu Kai-Yuan, Fu Man-Liang, Wang Hong-Ning, Gao Rong
Key Laboratory of Bio-Resource and Eco-Environment of Ministry Education, Bioengineering Research Center for Animal Disease Prevention and Control, Life Science College, Sichuan University, Chengdu, Sichuan, PR China.
Biotechnol J. 2008 Feb;3(2):264-73. doi: 10.1002/biot.200700050.
This study was conducted to explore the synergetic effect of a novel plasmid containing a porcine IL-6 gene and CpG motifs on immunity of mice in order to develop an effective adjuvant to boost resistance against infection. The synthetic oligodeoxynucleotide containing 11 CpG motifs was inserted into the reconstructed VR1020 plasmid containing the pig IL-6 gene (VRPIL6), designated VRIL6C, and then encapsulated in chitosan nanoparticles (CNP) prepared by ionic cross linkage, designated VRIL6C-CNP. The 3-week old mice were injected, respectively, with VRIL6C-CNP, VRIL6-CNP, CpG-CNP and VR1020-CNP to detect the changes of immunity. At 28 days post inoculation, the mice were challenged with virulent hemolytic serotype 2 Streptococcus to test their resistance against infection. The results showed that there was a significant increase in immunoglobulins and interleukins in mice receiving VRIL6C-CNP compared with the control groups, as well as an increase in the lymphocytes and monocytes in the inoculated mice, so that the immunity was remarkably improved in the VRIL6C-CNP group. The challenge provoked stronger immunity and protection against infection in the VRIL6C-CNP group than in the control mice that manifested severe symptoms and lesions. This suggests that VRIL6C-CNP could remarkably enhance the nonspecific immunity of mice, and facilitate the development of an effective immunopotentiator to promote the resistance of the animals against infection.
本研究旨在探索一种含有猪白细胞介素-6基因和CpG基序的新型质粒对小鼠免疫力的协同作用，以便开发一种有效的佐剂来增强抗感染能力。将含有11个CpG基序的合成寡脱氧核苷酸插入含有猪白细胞介素-6基因的重组VR1020质粒（VRPIL6）中，命名为VRIL6C，然后将其封装在通过离子交联制备的壳聚糖纳米颗粒（CNP）中，命名为VRIL6C-CNP。分别给3周龄的小鼠注射VRIL6C-CNP、VRIL6-CNP、CpG-CNP和VR1020-CNP，以检测免疫变化。接种后28天，用强毒溶血2型链球菌攻击小鼠，以测试它们的抗感染能力。结果显示，与对照组相比，接受VRIL6C-CNP的小鼠体内免疫球蛋白和白细胞介素显著增加，接种小鼠的淋巴细胞和单核细胞也增加，因此VRIL6C-CNP组的免疫力显著提高。与表现出严重症状和病变的对照小鼠相比，VRIL6C-CNP组在攻击后引发了更强的免疫力和抗感染保护作用。这表明VRIL6C-CNP可以显著增强小鼠的非特异性免疫力，并有助于开发一种有效的免疫增强剂来提高动物的抗感染能力。
