Lødrup Anders Bergh, Karstoft Kristian, Dissing Thomas Heide, Pedersen Michael, Nyengaard Jens Randel
Stereology and Electron Microscopy Research Laboratory and MIND Center, Aarhus University, Aarhus, Denmark.
Virchows Arch. 2008 Apr;452(4):393-403. doi: 10.1007/s00428-007-0520-6. Epub 2008 Jan 24.
In this study, we wanted to evaluate the use of kidney biopsies for estimation of N(glom) and V(glom) in both healthy and chronically diseased kidneys. Danish Landrace pigs with mean weight of 29 kg (range: 25-35 kg) were either subjected to unilateral ureteral obstruction (UUO) or non-obstruction (healthy). N(glom) and V(glom) was estimated by design-based methods using biopsies, N(glom)(biopsy) and V(glom)(biopsy). From each kidney, six biopsies were withdrawn at six topographically different sites. All estimates were done following stereological principles and reference methods estimated number with the physical fractionator, N(glom)(PF), and volume with test point system, V(glom)(TPS). N(glom)(PF) was 1.30 +/- 0.28 x 10(6) for UUO kidneys and 1.59 +/- 0.28 x 10(6) for healthy kidneys. N(glom)(biopsy) was 1.18 +/- 0.28 x 10(6) (p > 0.05) for UUO and 1.33 +/- 0.24 x 10(6) (p = 0.04) for healthy kidneys. When UUO and healthy kidneys were grouped, N(glom)(PF) was 1.46 +/- 0.31 x 10(6), and N(glom)(biopsy) was 1.28 +/- 0.25 x 10(6) (p > 0.05). V(glom)(TPS) was 1,079 +/- 126 mm(3) for UUO and 1,707 +/- 263 mm(3) for healthy kidneys. V(glom)(biopsy) was 1,048 +/- 291 mm(3) for UUO (p > 0.05) and 1,373 +/- 393 mm(3) for healthy kidneys (p > 0.05). When UUO and healthy kidneys were grouped, V(glom)(TPS) was 1,180 +/- 229 mm(3) and V(glom)(biopsy) 1,129 +/- 334 mm(3) (p > 0.05). Biopsy sites were tested for any systematic differences between site- and mean values, and no significant difference was found (p > 0.05). This study showed that biopsies can be used for estimating N(glom) and V(glom) by design-based methods, but more precise determination of biopsy volume is needed.
在本研究中，我们想要评估肾活检在估计健康和慢性疾病肾脏中的肾小球数量（N(glom)）和肾小球体积（V(glom)）方面的应用。平均体重29千克（范围：25 - 35千克）的丹麦长白猪，要么接受单侧输尿管梗阻（UUO），要么不梗阻（健康对照）。通过基于设计的方法，利用活检样本估计N(glom)和V(glom)，即N(glom)(活检)和V(glom)(活检)。从每个肾脏的六个不同地形部位取出六份活检样本。所有估计均遵循体视学原理，参考方法用物理分割器估计数量N(glom)(PF)，用测试点系统估计体积V(glom)(TPS)。UUO肾脏的N(glom)(PF)为1.30±0.28×10⁶，健康肾脏为1.59±0.28×10⁶。UUO肾脏的N(glom)(活检)为1.18±0.28×10⁶（p>0.05），健康肾脏为1.33±0.24×10⁶（p = 0.04）。当将UUO和健康肾脏分组时，N(glom)(PF)为1.46±0.31×10⁶，N(glom)(活检)为1.28±0.25×10⁶（p>0.05）。UUO肾脏的V(glom)(TPS)为1079±126立方毫米，健康肾脏为1707±263立方毫米。UUO肾脏的V(glom)(活检)为1048±291立方毫米（p>0.05），健康肾脏为1373±393立方毫米（p>0.05）。当将UUO和健康肾脏分组时，V(glom)(TPS)为1180±229立方毫米，V(glom)(活检)为1129±334立方毫米（p>0.05）。对活检部位的位点值与平均值之间的任何系统差异进行了测试，未发现显著差异（p>0.05）。本研究表明，活检可用于通过基于设计的方法估计N(glom)和V(glom)，但需要更精确地确定活检体积。
