Bokara Kiran Kumar, Brown Erika, McCormick Rashidi, Yallapragada Prabhakara Rao, Rajanna Sharada, Bettaiya Rajanna
Department of Zoology, Andhra University, Visakhapatnam, 530 003, India.
Biometals. 2008 Feb;21(1):9-16. doi: 10.1007/s10534-007-9088-5. Epub 2007 Feb 14.
Pregnant rats were treated with 0.4% lead acetate through drinking water from 6th day of gestation and this treatment was continued till 21 post natal days (PND). Four regions of the brain namely hippocampus, cerebellum, frontal cortex and brain stem were dissected at 10, 20, 30 and 40 PND for estimation of lipid peroxidation products (LPP), catalase (CAT) and superoxide dismutase (SOD). The results indicate that there was a significant (P < 0.05) increase of LPP in exposed rats than their corresponding control at 10, 20 and 30 PND both in hippocampus and cerebellum. At PND 40, the LPP of control and exposed were found to be almost same in both the tissues indicating recovery from lead toxicity. CAT activity was significantly (P < 0.05) high in hippocampus of exposed rats up to PND 30 but up to PND 20 in cerebellum and frontal cortex. However, in brain stem, a significant (P < 0.05) increase in CAT activity was observed only at PND 10. A significant (P < 0.05) increase in SOD activity was observed up to PND 30 both in hippocampus and cerebellum on lead exposure. Frontal cortex exhibited a similar significant (P < 0.05) increase of SOD activity up to PND 20 and for brain stem up to PND 10. There was no significant change in the activity of antioxidant enzymes (CAT and SOD) and LPP in all the four brain tissues of control and exposed rats at PND 40 indicating recovery from lead-induced oxidative stress.
从妊娠第6天开始，给怀孕大鼠通过饮用水给予0.4%的醋酸铅，这种处理持续到出生后21天（PND）。在出生后10、20、30和40天解剖大脑的四个区域，即海马体、小脑、额叶皮质和脑干，以评估脂质过氧化产物（LPP）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）。结果表明，在出生后10、20和30天，暴露组大鼠海马体和小脑的LPP均显著（P<0.05）高于相应对照组。在出生后40天，发现两个组织中对照组和暴露组的LPP几乎相同，表明从铅毒性中恢复。在出生后30天之前，暴露组大鼠海马体中的CAT活性显著（P<0.05）较高，但在小脑和额叶皮质中直到出生后20天较高。然而，在脑干中，仅在出生后10天观察到CAT活性显著（P<0.05）增加。铅暴露后，在出生后30天之前，海马体和小脑的SOD活性均显著（P<0.05）增加。额叶皮质在出生后20天之前SOD活性有类似的显著（P<0.05）增加，脑干在出生后10天之前有类似增加。在出生后40天，对照组和暴露组大鼠所有四个脑组织中的抗氧化酶（CAT和SOD）活性和LPP均无显著变化，表明从铅诱导的氧化应激中恢复。
