从加莱菲康和森诺菲康硅水凝胶隐形眼镜中提取的溶菌酶的构象和定量表征。
Conformational and quantitative characterization of lysozyme extracted from galyfilcon and senofilcon silicone hydrogel contact lenses.
作者信息
Glasier Mary-Ann, Keech Adam, Sheardown Heather, Subbaraman Lakshman N, Jones Lyndon
机构信息
Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada.
出版信息
Curr Eye Res. 2008 Jan;33(1):1-11. doi: 10.1080/02713680701830278.
PURPOSE
To compare two solvents for retrieval of lysozyme deposited on a silicone hydrogel (SH) contact lens material galyfilcon A (GA; Acuvue Advance).
METHODS
Two buffers used were 50:50 acetonitrile/0.02% trifluoroacetic acid (buffer 1) and 50:50 acetonitrile/50 mM NaOH (buffer 2).
RESULTS
Extraction efficiency from GA lenses was 74% (buffer 1) and 83% (buffer 2). Buffer 2 decreased lysozyme activity > buffer 1. Ex vivo GA lenses showed total protein deposition of 2-16 microg/lens with total lysozyme deposition of 0.3-3.9 microg/lens.
CONCLUSIONS
We have developed a low acid strength extraction buffer that can be used to efficiently extract active lysozyme protein from novel siloxane-based contact lens materials.
目的
比较两种用于从沉积在硅水凝胶（SH）隐形眼镜材料盖利菲康A（GA；爱尔康傲滴恒润）上的溶菌酶中进行提取的溶剂。
方法
使用的两种缓冲液分别是50:50乙腈/0.02%三氟乙酸（缓冲液1）和50:50乙腈/50 mM氢氧化钠（缓冲液2）。
结果
从GA镜片中的提取效率分别为74%（缓冲液1）和83%（缓冲液2）。缓冲液2使溶菌酶活性降低的程度大于缓冲液1。离体GA镜片显示每片镜片的总蛋白沉积量为2 - 16微克，总溶菌酶沉积量为0.3 - 3.9微克。
结论
我们开发了一种低酸强度提取缓冲液，可用于从新型硅氧烷基隐形眼镜材料中高效提取活性溶菌酶蛋白。
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