Glasier Mary-Ann, Keech Adam, Sheardown Heather, Subbaraman Lakshman N, Jones Lyndon
Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada.
Curr Eye Res. 2008 Jan;33(1):1-11. doi: 10.1080/02713680701830278.
To compare two solvents for retrieval of lysozyme deposited on a silicone hydrogel (SH) contact lens material galyfilcon A (GA; Acuvue Advance).
Two buffers used were 50:50 acetonitrile/0.02% trifluoroacetic acid (buffer 1) and 50:50 acetonitrile/50 mM NaOH (buffer 2).
Extraction efficiency from GA lenses was 74% (buffer 1) and 83% (buffer 2). Buffer 2 decreased lysozyme activity > buffer 1. Ex vivo GA lenses showed total protein deposition of 2-16 microg/lens with total lysozyme deposition of 0.3-3.9 microg/lens.
We have developed a low acid strength extraction buffer that can be used to efficiently extract active lysozyme protein from novel siloxane-based contact lens materials.
比较两种用于从沉积在硅水凝胶（SH）隐形眼镜材料盖利菲康A（GA；爱尔康傲滴恒润）上的溶菌酶中进行提取的溶剂。
使用的两种缓冲液分别是50:50乙腈/0.02%三氟乙酸（缓冲液1）和50:50乙腈/50 mM氢氧化钠（缓冲液2）。
从GA镜片中的提取效率分别为74%（缓冲液1）和83%（缓冲液2）。缓冲液2使溶菌酶活性降低的程度大于缓冲液1。离体GA镜片显示每片镜片的总蛋白沉积量为2 - 16微克，总溶菌酶沉积量为0.3 - 3.9微克。
我们开发了一种低酸强度提取缓冲液，可用于从新型硅氧烷基隐形眼镜材料中高效提取活性溶菌酶蛋白。
