Yin Ying, Wang Xiaorong, Sun Yuanyuan, Guo Hongyan, Yin Daqiang
State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210093, China.
Environ Toxicol. 2008 Jun;23(3):328-36. doi: 10.1002/tox.20330.
Laboratory experiments were carried out to investigate pyrene bioaccumulation and its consequent biological responses in submerged macrophyte Ceratophyllum demersum. Plants were exposed to different levels (0.01, 0.02, 0.05, 0.07, 0.1 mg/L) of pyrene for 10 days, and the pyrene content, and total free radicals in plant were analyzed. The pyrene concentration in plant was highly correlated to exposure concentration (R(2)=0.990). Electron paramagnetic resonance (EPR) analysis revealed that pyrene exposure significantly increased total free radicals in the plants. A strong positive correlation (R(2)=0.956) between O(2)(*-) generation and pyrene contents implied that pyrene exposure induced reactive oxygen species (ROS) and led to oxidative stress in C. demersum. The activities of antioxidant enzymes and the contents of glutathione were determined. Change in the contents of malondialdehyde (MDA) was also studied. Results indicated that the bioaccumulation of pyrene resulted in the changes of the antioxidant defense system and the production of ROS with the oxidative stress, ultimately induced damnification in C. demersum.
开展了实验室实验，以研究芘在沉水植物金鱼藻中的生物累积及其引发的生物学响应。将植物暴露于不同浓度（0.01、0.02、0.05、0.07、0.1 mg/L）的芘中10天，然后分析植物中的芘含量和总自由基。植物中的芘浓度与暴露浓度高度相关（R(2)=0.990）。电子顺磁共振（EPR）分析表明，芘暴露显著增加了植物中的总自由基。超氧阴离子（O(2)(*-)）生成与芘含量之间存在强正相关（R(2)=0.956），这表明芘暴露诱导了活性氧（ROS）的产生，并导致金鱼藻出现氧化应激。测定了抗氧化酶的活性和谷胱甘肽的含量。还研究了丙二醛（MDA）含量的变化。结果表明，芘的生物累积导致抗氧化防御系统发生变化，并伴随着氧化应激产生ROS，最终导致金鱼藻受到损伤。
