Yin Ying, Jia Haixia, Sun Yuanyuan, Yu Hongxia, Wang Xiaorong, Wu Jichun, Xue Yuqun
State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210093, People's Republic of China.
Comp Biochem Physiol C Toxicol Pharmacol. 2007 Mar;145(2):288-93. doi: 10.1016/j.cbpc.2007.01.002. Epub 2007 Jan 16.
In the present study, the bioaccumulation and reactive oxygen species (ROS) generation were studied after fish (Carassius auratus) were exposed to different concentrations (0.01, 0.02, 0.05, 0.07 and 0.1 mg/L) of phenanthrene for 4 days. The accumulation of phenanthrene in liver increased with the exposure concentration (R(2)=0.88). A secondary spin trapping technique was used followed by electron paramagnetic resonance (EPR) analysis, to study the ROS production. The ROS generated in fish liver after exposure to phenanthrene was identified as hydroxyl radical (()OH). The ()OH signal intensity of the EPR spectrum showed a significant increase (p<0.05) compared to the control when the phenanthrene concentration was as low as 0.05 mg/L. A good positive relationship (R(2)=0.97) was found between the ()OH formation and exposure concentrations. The changes of the activities of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), and contents of reduced glutathione (GSH) also were detected. The results clearly indicated that phenanthrene could induce ()OH generation and result in oxidative stress in liver of fish.
在本研究中，研究了将金鱼（Carassius auratus）暴露于不同浓度（0.01、0.02、0.05、0.07和0.1 mg/L）的菲中4天后的生物累积和活性氧（ROS）生成情况。肝脏中菲的累积量随暴露浓度的增加而增加（R(2)=0.88）。采用二次自旋捕获技术并结合电子顺磁共振（EPR）分析来研究ROS的产生。暴露于菲后在鱼肝脏中产生的ROS被鉴定为羟基自由基（()OH）。当菲浓度低至0.05 mg/L时，EPR光谱的()OH信号强度与对照组相比显著增加（p<0.05）。在()OH的形成与暴露浓度之间发现了良好的正相关关系（R(2)=0.97）。还检测了过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽-S-转移酶（GST）的活性变化以及还原型谷胱甘肽（GSH）的含量。结果清楚地表明，菲可诱导()OH的生成并导致鱼肝脏中的氧化应激。
