Yang Li, Shao Zhujun, Zhang Xiaobing, Xu Li, Peng Junping, Xu Xingye, Liang Xiaofeng, Qi Yipeng, Jin Qi
College of Life Sciences, Wuhan University, Wuhan, China.
J Infect. 2008 Mar;56(3):211-8. doi: 10.1016/j.jinf.2007.12.005. Epub 2008 Jan 22.
In China, comparatively little research has been directed at serogroup B meningococci, which are mainly isolated from healthy individuals. We attempted to study the genotypic characterization of Neisseria meningitidis serogroup B strains.
We analyzed 150 N. meningitidis strains isolated in China during 1975-2005 by multilocus sequence typing (MLST) and porA typing.
A total of 88 different sequence types (STs) were identified by MLST, 73 of which were newly identified. Seven complexes previously identified in other countries and three unique clonal lineages first identified in China were detected, seven of which had previously been described as 'hyperinvasive meningococcal lineages'. Several lineages were found in specified period. A total of 63 different porA types were found, 11 of which were novel. The most common porA types were P1.5-1,2-2 (17 isolates), P1.5-1,10-4 (12 isolates), P1.5-2,2-2 (eight isolates) and P1.7-2,4 (seven isolates).
In this context, serogroup B meningococci provide a diverse, continually reassorted gene pool from which new genotypes arise. The most important mechanism is probably horizontal genetic exchange among N. meningitidis serogroup B strains, possibly resulting in the emergence of new meningococcal clones. These results may help our understanding of the genotypic distribution of serogroup B meningococci and provide clues for further study of this organism.
在中国，针对主要从健康个体中分离出的B群脑膜炎奈瑟菌的研究相对较少。我们试图研究B群脑膜炎奈瑟菌菌株的基因特征。
我们通过多位点序列分型（MLST）和porA分型分析了1975年至2005年期间在中国分离出的150株脑膜炎奈瑟菌菌株。
通过MLST共鉴定出88种不同的序列类型（STs），其中73种是新鉴定出的。检测到先前在其他国家鉴定出的7个复合体和在中国首次鉴定出的3个独特克隆谱系，其中7个先前被描述为“高侵袭性脑膜炎球菌谱系”。在特定时期发现了几个谱系。共发现63种不同的porA类型，其中11种是新的。最常见的porA类型是P1.5-1,2-2（17株分离株）、P1.5-1,10-4（12株分离株）、P1.5-2,2-2（8株分离株）和P1.7-2,4（7株分离株）。
在这种情况下，B群脑膜炎奈瑟菌提供了一个多样化的、不断重新组合的基因库，从中产生新基因型。最重要的机制可能是B群脑膜炎奈瑟菌菌株之间的水平基因交换，这可能导致新的脑膜炎球菌克隆出现。这些结果可能有助于我们了解B群脑膜炎奈瑟菌的基因分型分布，并为进一步研究该生物体提供线索。
