Shim Youn-Young, Wanasundara Janitha P D
Saskatoon Research Centre, Agriculture and Agri-Food Canada, Saskatchewan, Canada.
J Agric Food Chem. 2008 Feb 27;56(4):1184-92. doi: 10.1021/jf072660u. Epub 2008 Jan 25.
Allergy to yellow mustard (YM; Sinapis alba L.) seed proteins has been reported and is currently seen as a constraint that hampers expansion of YM protein utilization. The most predominant allergenic protein of YM seed has been recognized as Sin a 1. In this study, Sin a 1 was purified ( S. alba var. Andante), rabbit polyclonal antibodies (pAb) specific to Sin a 1 were generated, and a sandwich enzyme-linked immunosorbent assay (S-ELISA) was developed to detect and quantify Sin a 1 from YM. The S-ELISA method using Sin a 1-pAb and its horseradish peroxidase conjugate resulted in a detection limit of 0.3 microg/mL for purified Sin a 1. The Sin a 1 contents of six YM lines were in the range of 0.82-2.94 mg/g when assayed by the developed S-ELISA method. The results showed that S-ELISA could distinguish Sin a 1 in YM seed-derived extracts rapidly and could be applied in controlling and/or monitoring of YM allergenic proteins.
对黄芥末（YM；白芥）种子蛋白过敏的情况已有报道，目前被视为阻碍黄芥末蛋白利用扩大的一个限制因素。黄芥末种子最主要的致敏蛋白已被确认为Sin a 1。在本研究中，纯化了Sin a 1（白芥变种Andante），制备了针对Sin a 1的兔多克隆抗体（pAb），并开发了一种夹心酶联免疫吸附测定法（S-ELISA）来检测和定量黄芥末中的Sin a 1。使用Sin a 1-pAb及其辣根过氧化物酶缀合物的S-ELISA方法对纯化的Sin a 1的检测限为0.3微克/毫升。通过开发的S-ELISA方法测定，六个黄芥末品系的Sin a 1含量在0.82-2.94毫克/克范围内。结果表明，S-ELISA能够快速区分黄芥末种子提取物中的Sin a 1，可用于控制和/或监测黄芥末致敏蛋白。
