大肠杆菌（m5U54）-甲基转移酶在tRNA-琼脂糖上的亲和层析
Affinity chromatography of Escherichia coli (m5U54)-methyltransferase on tRNA-agarose.
作者信息
Gu X, Santi D V
机构信息
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
出版信息
Protein Expr Purif. 1991 Feb;2(1):66-8. doi: 10.1016/1046-5928(91)90012-8.
PMID:1821774
Abstract
tRNA-agarose was prepared by condensing periodate-oxidized tRNA to an agarose matrix containing hydrazide functional groups. The tRNA-agarose was used to take partially purified tRNA (m5U54)-methyltransferase to homogeneity. The method is simple and reproducible and gives high yields.
摘要
通过将高碘酸盐氧化的tRNA缩合到含有酰肼官能团的琼脂糖基质上来制备tRNA-琼脂糖。tRNA-琼脂糖用于将部分纯化的tRNA（m5U54）-甲基转移酶纯化至均一。该方法简单且可重复，产率高。
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