Zhu Qi, Hong Ailing, Sheng Nijing, Zhang Xiaolin, Matejko Anna, Jun Kyu-Yeon, Srivannavit Onnop, Gulari Erdogan, Gao Xiaolian, Zhou Xiaochuan
Department of Biology and Biochemistry, University of Houston, TX, USA.
Methods Mol Biol. 2007;382:287-312. doi: 10.1007/978-1-59745-304-2_19.
We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using a microRNA detection chip, (2) protein binding and function analysis using epitope, kinase substrate, or phosphopeptide chips, and (3) protein-binding analysis using oligonucleotide chips. These diverse categories of customizable arrays are based on the same biochip platform featuring a significant amount of flexibility in the sequence design to suit a wide range of research needs. The protocols of the array applications play a critical role in obtaining high quality and reliable results. Given the comprehensive and complex nature of the array experiments, the details presented in this chapter is intended merely as a useful information source of reference or a starting point for many researchers who are interested in genome- or proteome-scale studies of proteins and nucleic acids and their interactions.
在本章中，我们将介绍基于微流控芯片的寡核苷酸或肽微阵列（阵列）的应用。具体而言，将展示三个主要应用：（1）使用microRNA检测芯片检测microRNA/小RNA；（2）使用表位、激酶底物或磷酸肽芯片进行蛋白质结合和功能分析；（3）使用寡核苷酸芯片进行蛋白质结合分析。这些不同类型的可定制阵列基于同一生物芯片平台，该平台在序列设计方面具有显著的灵活性，以满足广泛的研究需求。阵列应用的实验方案对于获得高质量和可靠的结果起着关键作用。鉴于阵列实验的全面性和复杂性，本章所呈现的细节仅旨在作为一个有用的参考信息来源，或为许多对蛋白质和核酸及其相互作用进行基因组或蛋白质组规模研究感兴趣的研究人员提供一个起点。
