Hutton Jonathon F, D'Andrea Richard J, Lewis Ian D
Division of Haematology and Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, 5000, South Australia.
Curr Stem Cell Res Ther. 2007 Sep;2(3):229-37. doi: 10.2174/157488807781696221.
The establishment of culture systems that promote haemopoietic stem cell (HSC) self-renewal and expansion ex vivo will increase the clinical potential of umbilical cord blood (CB) HSC transplantation. Studies defining key signalling pathways that regulate development and expansion of HSC in vivo have greatly facilitated development of protocols for expanding HSC in ex vivo culture. Recently a number of soluble factors with novel stem cell expansion activity have been identified as part of pathways associated with mesodermal induction, or as factors produced by supportive stroma. These have been reported to support, to varying degrees, HSC self-renewal under in vitro conditions. Here we review the activities of these new factors and consider their future potential as components in ex vivo expansion culture for CB HSC. Finally we discuss the challenges associated with applying these factors to clinically relevant culture systems.
建立能够促进造血干细胞（HSC）体外自我更新和扩增的培养体系，将提高脐带血（CB）造血干细胞移植的临床应用潜力。确定体内调节造血干细胞发育和扩增的关键信号通路的研究，极大地推动了体外培养扩增造血干细胞方案的发展。最近，一些具有新型干细胞扩增活性的可溶性因子已被鉴定为中胚层诱导相关信号通路的一部分，或被鉴定为支持性基质产生的因子。据报道，这些因子在体外条件下能不同程度地支持造血干细胞的自我更新。在此，我们综述这些新因子的活性，并探讨它们作为CB造血干细胞体外扩增培养成分的未来潜力。最后，我们讨论将这些因子应用于临床相关培养体系所面临的挑战。
