亚甲基四氢叶酸还原酶677 T等位基因可预防西非地区的持续性乙肝病毒感染。

Methylenetetrahydrofolate reductase 677 T allele protects against persistent HBV infection in West Africa.

作者信息

Bronowicki Jean-Pierre, Abdelmouttaleb Idrissia, Peyrin-Biroulet Laurent, Venard Véronique, Khiri Hacène, Chabi Nicodème, Amouzou Emile K, Barraud Hélène, Halfon Philippe, Sanni Ambaliou, Bigard Marc-André, Le Faou Alain, Guéant Jean-Louis

机构信息

Inserm U724, Cellular and Molecular Pathology in Nutrition, Faculté de Médecine, University Henri Poincaré of Nancy, Vandoeuvre lès Nancy, France.

出版信息

J Hepatol. 2008 Apr;48(4):532-9. doi: 10.1016/j.jhep.2007.11.017. Epub 2008 Jan 2.

DOI:10.1016/j.jhep.2007.11.017

PMID:18222012

Abstract

BACKGROUND/AIMS: Homocysteine metabolism is linked to DNA methylation, a mechanism potentially involved in the course of hepatitis B virus (HBV) infection. We evaluated the association of determinants of homocysteine metabolism with the outcome of HBV infection.

METHODS

Four hundred and fifty-five healthy adults from Togo and Benin were tested for HBV serologic markers, HLA DR alleles, folate, vitamin B12, methylenetetrahydrofolate reductase (MTHFR) 677 C-->T, 1298 A-->C and methionine synthase 2756 A-->G polymorphisms.

RESULTS

Seventy-eight percent of the study population was anti-HBc positive. Among them, 202 (56.9%) were anti-HBs positive and 58 (16.3%) were HBsAg positive. After stepwise logistic regression, the MTHFR 677 T allele was independently associated with persistence of detectable anti-HBs antibodies (OR: 2.47; 95% CI: 1.29-4.71; p=0.006). The mean HBV DNA level was significantly lower in HBsAg positive subjects carrying the 677 T allele than in those with the 677 CC genotype (1000+/-1406 vs. 2,400,000+/-214,000 copies/ml, p=0.005). Beninese origin and HLA-DRB1*09 allele were the other determinants independently associated with favorable outcome of HBV infection.

CONCLUSIONS

The methylenetetrahydrofolate reductase 677 T allele seems to protect against chronic HBV infection in young African adults.

摘要

背景/目的：同型半胱氨酸代谢与DNA甲基化相关，这是一种可能参与乙型肝炎病毒（HBV）感染过程的机制。我们评估了同型半胱氨酸代谢决定因素与HBV感染结局的关联。

方法

对来自多哥和贝宁的455名健康成年人进行了HBV血清学标志物、HLA DR等位基因、叶酸、维生素B12、亚甲基四氢叶酸还原酶（MTHFR）677 C→T、1298 A→C以及甲硫氨酸合酶2756 A→G多态性检测。

结果

78%的研究人群抗-HBc呈阳性。其中，202人（56.9%）抗-HBs呈阳性，58人（16.3%）HBsAg呈阳性。经过逐步逻辑回归分析，MTHFR 677 T等位基因与可检测到的抗-HBs抗体持续存在独立相关（比值比：2.47；95%置信区间：1.29 - 4.71；p = 0.006）。携带677 T等位基因的HBsAg阳性受试者的平均HBV DNA水平显著低于携带677 CC基因型的受试者（1000±1406对2,400,000±214,000拷贝/毫升，p = 0.005）。贝宁血统和HLA-DRB1*09等位基因是与HBV感染良好结局独立相关的其他决定因素。