Amari J V, Brown P R, Pivarnik P E, Sehgal R K, Turcotte J G
Department of Chemistry, University of Rhode Island, Kingston 02881.
J Pharm Biomed Anal. 1991;9(10-12):871-5. doi: 10.1016/0731-7085(91)80016-3.
A reversed-phase chromatographic method is described for the analysis of an experimental anti-AIDS drug 3'-azido-3'-deoxythymidine monophosphate diglyceride (AZT-MP-DG) [J.M. Steim et al., Biochem. Biophys. Res. Commun. 171, 458-464 (1990)] [1], a phosphatidic acid derivative of AZT. Analytical conditions were based upon conventional separations of glycerophospholipid species. Where AZT-MP-DG was monitored by UV absorption, there were two wavelength maxima. The response was linear in the concentration range used in this study. The peak was characterized by absorbance ratios with a rapid scanning UV detector.
描述了一种反相色谱法，用于分析一种实验性抗艾滋病药物3'-叠氮-3'-脱氧胸苷单磷酸二甘油酯（AZT-MP-DG）[J.M. 施泰姆等人，《生物化学与生物物理研究通讯》171, 458 - 464 (1990)] [1]，AZT的一种磷脂酸衍生物。分析条件基于甘油磷脂种类的常规分离。当通过紫外吸收监测AZT-MP-DG时，有两个最大波长。在本研究使用的浓度范围内，响应呈线性。该峰通过使用快速扫描紫外检测器的吸光度比来表征。
