Telting-Diaz M, Kelly M T, Hua C, Smyth M R
School of Chemical Sciences, Dublin City University, Ireland.
J Pharm Biomed Anal. 1991;9(10-12):889-93. doi: 10.1016/0731-7085(91)80019-6.
The electrochemical properties of the calcium-channel blockers, nifedipine and nicardipine, and the beta-blocking agent, pindolol, have been exploited for the determination of their concentrations in plasma samples. High-performance liquid chromatography (HPLC) separation was carried out on a cyanopropyl modified column and the drugs were detected in a flow-through carbon fibre microelectrode cell. The chromatographic system was coupled to a column-switching arrangement in order to perform on-line solid-phase extraction of the drugs from spiked human plasma. Preliminary investigations showed the response of the method to be linear over a range of 20-500 ng ml-1 in plasma with a limit of detection of approximately 15 ng ml-1 for each compound.
钙通道阻滞剂硝苯地平与尼卡地平以及β受体阻滞剂吲哚洛尔的电化学性质已被用于测定它们在血浆样本中的浓度。在氰丙基改性柱上进行高效液相色谱（HPLC）分离，并在流通式碳纤维微电极池中检测药物。色谱系统与柱切换装置相连，以便从加标的人血浆中对药物进行在线固相萃取。初步研究表明，该方法在血浆中20 - 500 ng ml-1范围内响应呈线性，每种化合物的检测限约为15 ng ml-1。
