金属免疫分析中有机金属标记链霉亲和素 - 生物素系统的潜力

Potentiality of an organometallic labelled streptavidin--biotin system in metalloimmunoassay.

作者信息

Rémy I, Brossier P, Lavastre I, Besançon J, Moïse C

机构信息

Unité d'Immunoanalyse, Université de Bourgogne, Dijon, France.

出版信息

J Pharm Biomed Anal. 1991;9(10-12):965-7. doi: 10.1016/0731-7085(91)80031-4.

DOI:10.1016/0731-7085(91)80031-4

PMID:1822220

Abstract

Biotin labelled with a cymantrene moiety (cyclopentadienyl manganese tricarbonyl complex) is described for the first time. Because this metallo-biotin retains full recognition for the specific glycoprotein avidin (or streptavidin), the labelled streptavidin-biotin system is proposed for use in a solid-phase competition-type metalloimmunoassay in which bovine serum albumin (BSA) is used as a model of applications. Atomic absorption spectrometry is utilized for the detection of the cymantrene-labelled biotin.

摘要

首次描述了用 cymantrene 部分（环戊二烯基锰三羰基配合物）标记的生物素。由于这种金属化生物素对特异性糖蛋白抗生物素蛋白（或链霉抗生物素蛋白）仍具有完全识别能力，因此提出将标记的链霉抗生物素蛋白 - 生物素系统用于以牛血清白蛋白（BSA）作为应用模型的固相竞争型金属免疫测定中。利用原子吸收光谱法检测 cymantrene 标记的生物素。