Lee Hyun Sook, Cho Yona, Lee Jung-Hyun, Kang Sung Gyun
Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600, Republic of Korea.
J Bacteriol. 2008 Apr;190(7):2629-32. doi: 10.1128/JB.01722-07. Epub 2008 Jan 25.
The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In this study, we functionally assessed for the first time the monofunctional DDDD-type HolPase, which is organized in the gene cluster.
TON_0887基因被鉴定为嗜热古菌“嗜热栖热菌”NA1中缺失的组氨醇磷酸磷酸酶(HolPase)。该蛋白含有磷酸水解酶DDDD超家族的保守基序,重组表达的蛋白表现出很强的HolPase活性。在本研究中,我们首次对基因簇中组织的单功能DDDD型HolPase进行了功能评估。