Puymirat J, Miehe M, Marchand R, Sarlieve L, Dussault J H
Laboratoire d'Ontogénèse et de Génétique Moléculaire, CHU Laval, Sainte-Foy, Québec, Canada.
Thyroid. 1991;1(2):173-84. doi: 10.1089/thy.1991.1.173.
It is generally accepted that thyroid hormones act at the genomic level through an interaction with specific nuclear receptors. Using a monoclonal antibody raised against the rat liver nuclear L-T3 receptor (NTR), we report here the immunocytochemical localization of T3 receptors in the adult rat brain. The strongest NTR immunoreactivity was found in the olfactory bulb, the hippocampus, the dentate gyrus, the amygdala areas, and the neocortex (layers III-VI). An intermediate NTR immunoreactivity was found in the hypothalamus, whereas the thalamus, the caudate-putamen, and the pallidum were weakly NTR-immunoreactive. In the cerebellum, a strong NTR immunoreactivity was found in the nuclei of Purkinje cells, in the internal granular layer, and in some nuclei of cells located in the molecular layer. In the brainstem, a strong NTR immunoreactivity was found in the lateral mamillary nucleus and the interstitial nucleus. A weak to moderate NTR immunoreactivity was observed in the central gray matter, while the substantia nigra and the interpeduncular nucleus were weakly stained. Furthermore, we also found NTR immunoreactivity in the nuclei of ependymocytes, epithelial cells of the choroid plexus, and cells located in the white matter. At the electron microscope level, we confirm that the immunoreactivity was not only localized in the nuclei of neurons but also in the nuclei of astrocytes and medium oligodendrocytes. This study provides new information concerning the distribution of NTR in the rat brain: (1) NTR are present not only in neurons but also in glial and ependymal cells, and (2) there is a regional and cellular heterogeneity in the distribution of NTR in the central nervous system.
一般认为，甲状腺激素通过与特定核受体相互作用在基因组水平发挥作用。我们使用针对大鼠肝细胞核L-T3受体（NTR）产生的单克隆抗体，在此报告成年大鼠脑中T3受体的免疫细胞化学定位。在嗅球、海马体、齿状回、杏仁核区域和新皮质（III-VI层）发现最强的NTR免疫反应性。在下丘脑发现中等强度的NTR免疫反应性，而丘脑、尾状核-壳核和苍白球的NTR免疫反应性较弱。在小脑中，在浦肯野细胞的细胞核、内颗粒层以及分子层中的一些细胞核中发现强NTR免疫反应性。在脑干中，在外侧乳头体核和间质核中发现强NTR免疫反应性。在中央灰质中观察到弱至中等强度的NTR免疫反应性，而黑质和脚间核染色较弱。此外，我们还在室管膜细胞、脉络丛上皮细胞和白质中的细胞的细胞核中发现了NTR免疫反应性。在电子显微镜水平，我们证实免疫反应性不仅定位于神经元的细胞核，还定位于星形胶质细胞和中等少突胶质细胞的细胞核。本研究提供了有关大鼠脑中NTR分布的新信息：（1）NTR不仅存在于神经元中，还存在于神经胶质细胞和室管膜细胞中；（2）中枢神经系统中NTR的分布存在区域和细胞异质性。
