Salazar Edith L, Calzada Leobardo
Medical Research Unit in Endocrine Disease, Medical Research Coordination, Social Security Mexican Institute (IMSS), Mexico City, Mexico.
Gynecol Endocrinol. 2008 Jan;24(1):30-2. doi: 10.1080/09513590701673692.
We have studied the modulation and differential sensitivity of the estrogen receptor (ER) in breast cancer in the presence of protecting or modifying agents of disulfide bonds and sulfhydryl groups present in the ER steroid-binding domain. Protecting agents such as mercaptoethanol and dithiotreitol increased the [(3)H]estradiol binding to ER by 25% and 50%, respectively. Modifying agents such as p-chloromercuribenzensulfonate decreased the [(3)H]estradiol binding by 70% and this was nearly completely abolished by N-ethylmaleimide (94%). These data indicate that disulfide bonds and sulfhydryl groups in the steroid-binding domain are intimately involved in the maintenance of a receptor structure necessary for estradiol binding.
我们研究了在雌激素受体（ER）类固醇结合域中存在的二硫键和巯基的保护或修饰剂存在的情况下，乳腺癌中雌激素受体（ER）的调节和差异敏感性。诸如巯基乙醇和二硫苏糖醇等保护剂分别使[³H]雌二醇与ER的结合增加了25%和50%。诸如对氯汞苯磺酸盐等修饰剂使[³H]雌二醇结合减少了70%，而N-乙基马来酰亚胺（94%）几乎完全消除了这种减少。这些数据表明，类固醇结合域中的二硫键和巯基密切参与维持雌二醇结合所需的受体结构。
