缓激肽、ATP和肾上腺素对犬肾Madin-Darby细胞细胞膜电阻的影响。
Effect of bradykinin, ATP and adrenaline on cell membrane resistances of Madin-Darby canine kidney cells.
作者信息
Ritter M, Lang F
机构信息
Institute for Physiology, University of Innsbruck, Austria.
出版信息
J Physiol. 1991 Nov;443:45-54. doi: 10.1113/jphysiol.1991.sp018821.
Abstract
- Previous studies have shown that bradykinin, ATP and adrenaline hyperpolarize the cell membrane of Madin-Darby canine kidney (MDCK) cells by activation of calcium-sensitive K+ channels. The present study has been performed to determine the effect of these hormones on the resistance of the cell membrane and the cellular coupling. To this end, cellular cable analysis has been performed. 2. As a result, all three hormones lead to the expected, marked decrease of cell membrane resistance. 3. However, the bradykinin-induced reduction of cell membrane resistance was sustained, contrasting with only transient hyperpolarization induced by bradykinin and only transient activation of the K+ channels. Thus, the cable analysis reveals the sustained activation of an additional conductance. 4. ATP, but not the other two hormones, leads to a delayed increase of the intercellular coupling resistances. 5. Prolonged exposure of the cells to adrenaline leads to oscillations of the cell membrane potential, apparently by oscillatory activation of the K+ channels.
摘要
- 先前的研究表明，缓激肽、三磷酸腺苷（ATP）和肾上腺素通过激活钙敏感性钾离子通道，使犬肾传代细胞（MDCK）的细胞膜超极化。本研究旨在确定这些激素对细胞膜电阻和细胞耦联的影响。为此，进行了细胞电缆分析。2. 结果，所有这三种激素均导致细胞膜电阻出现预期的显著降低。3. 然而，缓激肽引起的细胞膜电阻降低是持续性的，这与缓激肽仅引起短暂的超极化以及仅短暂激活钾离子通道形成对比。因此，电缆分析揭示了另一种电导的持续激活。4. ATP会导致细胞间耦联电阻延迟增加，而其他两种激素则不会。5. 细胞长时间暴露于肾上腺素会导致细胞膜电位振荡，这显然是由钾离子通道的振荡激活引起的。
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