Sarath Vasiraju J, So Chang-Sok, Won Young Doo, Gollapudi Sastry
Division of Basic and Clinical Immunology, University of California, Irvine, CA, 92697, USA.
Anticancer Res. 2007 Nov-Dec;27(6B):3891-8.
Dried leaves of Artemisia princeps var orientalis are used in the Eastern practice of moxibustion to improve general health. The ability of A. princeps smoke and water extracts to induce apoptosis was evaluated in human breast cancer MCF-7 cells in vitro.
Tumor cells were cultured with a smoke or water extract (1.5-50% v/v) for 72 h, and cytotoxicity and apoptosis were determined by MTT and TUNEL assays, respectively. Activation of caspases, changes in membrane potential, and BCL-2 expression were determined by flow cytometry.
Both preparations inhibited the growth of breast cancer cells in a dose-dependent- manner. Induction of apoptosis was associated with activation of caspases 3, 8 and 9, depolarization of the mitochondrial membrane potential and down-regulation of BCL-2 expression. Furthermore, A. princeps smoke exerted synergistic cytotoxicity with doxorubicin.
The data suggest that A. princeps smoke and water soluble extracts induce apoptosis via the mitochondrial pathway and may represent a novel adjuvant for the treatment of breast cancer.
在东方医学实践中，艾叶干燥叶被用于艾灸以改善整体健康。本研究在体外评估了艾叶烟雾提取物和水提取物诱导人乳腺癌MCF-7细胞凋亡的能力。
将肿瘤细胞与烟雾提取物或水提取物（1.5 - 50% v/v）培养72小时，分别通过MTT法和TUNEL法测定细胞毒性和凋亡情况。通过流式细胞术测定半胱天冬酶的激活、膜电位变化和BCL-2表达。
两种提取物均以剂量依赖性方式抑制乳腺癌细胞的生长。凋亡诱导与半胱天冬酶3、8和9的激活、线粒体膜电位的去极化以及BCL-2表达的下调有关。此外，艾叶烟雾提取物与阿霉素具有协同细胞毒性作用。
数据表明，艾叶烟雾提取物和水溶性提取物通过线粒体途径诱导凋亡，可能代表一种治疗乳腺癌的新型佐剂。
