Schmidt D, Steffen H, Planta C
Biochim Biophys Acta. 1976 Aug 4;443(1):1-9. doi: 10.1016/0005-2736(76)90486-7.
Lateral diffusion coefficient and order parameter measurements were made with pyrene excimer optical probes and fatty acid spin label probes respectively in pure dipalmitoyl phosphatidylcholine membranes and in membranes doped with tocopheryl acetate. The investigation shows, that the lateral diffusion coefficient for pyrene in dipalmitoyl pholphatidylcholine membranes is decreased whereas the order parameter of the fatty acid chains is slightly increased in the inner part of the membranes by the addition of tocopheryl acetate. The fluid-solid equilibrium phase diagram of dipalmitoyl phosphatidylcholine/tocopheryl acetate mixed membranes has been constructed from the measurements of the partition of (2,2,6,6-tetramethylpiperdine-1-oxyl) TEMPO spin labels between lipid and aqueous regions as function of temperature. In the membranes tocopheryl acetate induces a strong broadening of the temperature range of the phase transition. At low tocopheryl acetate concentrations dipalmitoyl phosphatidylcholine and tocopheryl acetate seem to be completely miscible in the solid and in the liquid crystalline state.
分别使用芘准分子光学探针和脂肪酸自旋标记探针，在纯二棕榈酰磷脂酰胆碱膜以及掺杂了醋酸生育酚的膜中进行横向扩散系数和序参数测量。研究表明，在二棕榈酰磷脂酰胆碱膜中，芘的横向扩散系数降低，而通过添加醋酸生育酚，膜内部脂肪酸链的序参数略有增加。根据（2,2,6,6 - 四甲基哌啶 - 1 - 氧基）TEMPO自旋标记在脂质和水相区域之间的分配随温度的测量结果，构建了二棕榈酰磷脂酰胆碱/醋酸生育酚混合膜的液 - 固平衡相图。在膜中，醋酸生育酚使相变的温度范围显著变宽。在低醋酸生育酚浓度下，二棕榈酰磷脂酰胆碱和醋酸生育酚在固态和液晶态似乎完全互溶。
