Kim Song-Bae, Park Seong-Jik, Lee Chang-Gu, Choi Nag-Choul, Kim Dong-Ju
Environmental Biocolloid Engineering Laboratory, Program in Rural System Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
Colloids Surf B Biointerfaces. 2008 Jun 1;63(2):236-42. doi: 10.1016/j.colsurfb.2007.12.003. Epub 2007 Dec 14.
This study investigated the transport of bacteria through goethite-coated sand, focusing on the effects of solution pH and coated sand content on the transport of Escherichia coli ATCC 11105. The first set of column experiments was performed in columns (length 30 cm, diameter 5 cm) packed with quartz sand coated with goethite in solution having a pH in the range of 6-9. The second was carried out in columns (length 30 cm, diameter 2.5 cm) with varying coated sand contents ranging from 0 to 100%. Results indicate that the bacteria transport in the coated sand was influenced by solution pH. Around pH 6 and 7, bacterial mass recoveries were low at 2.4-6.7% while they were high at 76.3-81.6% around pH 8 and 9. Around pH 8, the positively charged coated sand may convert to being negatively charged, causing an electrostatically repulsive interaction between the coated sand and bacteria, thus effecting a sharp change in the mass recovery. Results also reveal that the mass recovery decreased from 76.7 to 2.7% as the coated sand content increased from 0 to 100%, showing the nonlinear dependency of mass recovery on the content of coated sand. This study demonstrates the importance of the solution pH and coated sand content in the adhesion of bacteria to goethite-coated sand and furthermore contributes to the knowledge of bacterial removal in positively charged porous media.
本研究调查了细菌在针铁矿包覆砂中的运移情况,重点关注溶液pH值和包覆砂含量对大肠杆菌ATCC 11105运移的影响。第一组柱实验在填充有针铁矿包覆石英砂的柱体(长度30 cm,直径5 cm)中进行,溶液pH值范围为6至9。第二组实验在包覆砂含量从0到100%变化的柱体(长度30 cm,直径2.5 cm)中开展。结果表明,包覆砂中细菌的运移受溶液pH值影响。在pH值约为6和7时,细菌质量回收率较低,为2.4%至6.7%,而在pH值约为8和9时则较高,为76.3%至81.6%。在pH值约为8时,带正电的包覆砂可能转变为带负电,导致包覆砂与细菌之间产生静电排斥相互作用,从而使质量回收率发生急剧变化。结果还显示,随着包覆砂含量从0增加到100%,质量回收率从76.7%降至2.7%,表明质量回收率对包覆砂含量呈非线性依赖关系。本研究证明了溶液pH值和包覆砂含量在细菌与针铁矿包覆砂黏附中的重要性,并且进一步增进了对带正电多孔介质中细菌去除的认识。