Ong Ping-Lin, Weng Bor-Chun, Lu Fung-Jou, Lin Miao-Ling, Chang Ting-Ting, Hung Ray-Ping, Chen Ching-Hsein
Department of Biochemical Science and Technology, College of Life Sciences, National Chiayi University, Chiayi, Taiwan.
Food Chem Toxicol. 2008 May;46(5):1535-47. doi: 10.1016/j.fct.2007.12.015. Epub 2007 Dec 23.
Bidens alba has been used for healing cuts, injuries, swellings, hypertension, jaundice, and diabetes in some countries. However, the effect of B. alba on human cancer remains poorly understood. The goal of this study was to investigate whether B. alba protein-extract could have an anticancer property against human colorectal cancer. The human colorectal cancer SW 480 cells treated with the protein-extract of B. alba would cause marked DNA damages and apoptosis-related cellular morphologies. Treatment with 225 microg/ml B. alba protein-extract also led to the SW480 cells to produce readily intracellular reactive oxygen species (ROS) after 1h of treatment and last to 24 h. The intracellular glutathione (GSH) depletion occurred after 12-24h of treatment. The treatment of the protein-extract would also caused mitochondrial transmembrane potential (DeltaPsi(m)) to decrease and cytosolic cytochrome c to increase. The caspase 3/7 activities were activated from 3 to 6 h after the treatment. The percentages of apoptosis induced by the protein-extract of B. alba decreased 26.4%, 10.1%, and 29.4% when the SW 480 cells were pretreated with Vitamin C, N-acetylcysteine, and Boc-Asp(OMe)-fmk, respectively. Taken together, we demonstrated for the first time that the protein-extract of B. alba could induce apoptosis that was related to the ROS production and GSH depletion in human colorectal cancer. The protein-extract of B. alba might have therapeutic value against the human colorectal cancer.
在一些国家，白花鬼针草已被用于治疗伤口、损伤、肿胀、高血压、黄疸和糖尿病。然而，白花鬼针草对人类癌症的影响仍知之甚少。本研究的目的是调查白花鬼针草蛋白提取物是否对人类结直肠癌具有抗癌特性。用白花鬼针草蛋白提取物处理的人类结直肠癌SW 480细胞会导致明显的DNA损伤和凋亡相关的细胞形态变化。用225微克/毫升白花鬼针草蛋白提取物处理1小时后，SW480细胞也会迅速产生细胞内活性氧（ROS），并持续到24小时。处理12 - 24小时后细胞内谷胱甘肽（GSH）耗尽。蛋白提取物的处理还会导致线粒体跨膜电位（ΔΨm）降低和胞质细胞色素c增加。处理后3至6小时激活半胱天冬酶3/7活性。当SW 480细胞分别用维生素C、N - 乙酰半胱氨酸和Boc - Asp(OMe)-fmk预处理时，白花鬼针草蛋白提取物诱导的凋亡百分比分别降低了26.4%、10.1%和29.4%。综上所述，我们首次证明白花鬼针草蛋白提取物可诱导与人类结直肠癌中ROS产生和GSH消耗相关的凋亡。白花鬼针草蛋白提取物可能对人类结直肠癌具有治疗价值。
