体外有丝分裂纺锤体组装
Mitotic spindle assembly in vitro.
作者信息
Merlie J, Heald R
机构信息
University of California, Berkeley, Berkeley, Carolina, USA.
出版信息
Curr Protoc Cell Biol. 2001 May;Chapter 11:Unit 11.13. doi: 10.1002/0471143030.cb1113s09.
PMID:18228304
Abstract
The protocols in this unit describe the preparation of materials for an in vitro assay of mitotic spindle assembly in Xenopus egg extracts. Fluorochrome-labeled tubulin is used to visualize microtubule asters and spindles.
摘要
本单元中的实验方案描述了用于非洲爪蟾卵提取物有丝分裂纺锤体组装体外测定的材料制备方法。荧光染料标记的微管蛋白用于观察微管星状体和纺锤体。
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