在纯化和半纯化系统中对真核生物翻译的分析。
Analysis of eukaryotic translation in purified and semipurified systems.
作者信息
Merrick W C, Hensold J O
机构信息
Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
出版信息
Curr Protoc Cell Biol. 2001 May;Chapter 11:Unit 11.9. doi: 10.1002/0471143030.cb1109s08.
PMID:18228314
Abstract
Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of intermediates in translation initiation and the roles of the translation factors in vitro.
摘要
正如本文所述，目前对翻译起始过程中连续步骤的许多理解是通过使用蔗糖梯度来分离核糖体和核糖体亚基而获得的。这些纯化的组分与纯化的翻译因子相结合，以分析翻译起始中间体的形成以及翻译因子在体外的作用。
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