Hawes C, Brandizzil F, Batoko H, Moore I
Oxford Brookes University, Oxford, United Kingdom.
Curr Protoc Cell Biol. 2001 May;Chapter 13:Unit 13.3. doi: 10.1002/0471143030.cb1303s09.
By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems in leaf tissue to permit visualization of ER and Golgi dynamics in vivo by epifluorescence or confocal microscopy.
通过添加植物或哺乳动物靶向序列，绿色荧光蛋白（GFP）可被导向植物细胞中的内质网、高尔基体或这两种细胞器。本单元描述了快速农杆菌和病毒介导的瞬时表达系统在叶片组织中的应用，以便通过落射荧光或共聚焦显微镜在体内观察内质网和高尔基体的动态变化。
