植物细胞中的细胞器运动：利用绿色荧光蛋白成像高尔基体和内质网动态

Organelle motility in plant cells: imaging golgi and ER dynamics with GFP.

作者信息

Hawes C, Brandizzil F, Batoko H, Moore I

机构信息

Oxford Brookes University, Oxford, United Kingdom.

出版信息

Curr Protoc Cell Biol. 2001 May;Chapter 13:Unit 13.3. doi: 10.1002/0471143030.cb1303s09.

DOI:10.1002/0471143030.cb1303s09

PMID:18228322

Abstract

By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems in leaf tissue to permit visualization of ER and Golgi dynamics in vivo by epifluorescence or confocal microscopy.

摘要

通过添加植物或哺乳动物靶向序列，绿色荧光蛋白（GFP）可被导向植物细胞中的内质网、高尔基体或这两种细胞器。本单元描述了快速农杆菌和病毒介导的瞬时表达系统在叶片组织中的应用，以便通过落射荧光或共聚焦显微镜在体内观察内质网和高尔基体的动态变化。