Overview of cell fractionation.
作者信息
Castle J D
机构信息
University of Virginia, Charlottesville, Virginia, USA.
出版信息
Curr Protoc Cell Biol. 2001 May;Chapter 3:Unit 3.1. doi: 10.1002/0471143030.cb0301s00.
PMID:18228352
Abstract
Cell fractionation is a useful preparative and analytical method in cell biology. It is essential for analysis of composition and function of cellular compartments and it is used to prepare materials for in vitro reconstitution studies This overview discusses the basic principles of centrifugation, the instruments available, choice of media, evaluation of fractionation, and procedure optimization.
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