Brown W J
Cornell University, Ithaca, New York, USA.
Curr Protoc Cell Biol. 2001 May;Chapter 4:Unit 4.6. doi: 10.1002/0471143030.cb0406s01.
This carefully written unit describes two methods for using the immunoperoxidase reaction to localize antigens at the electron microscope level; one for adherent cultured cells and one for tissue sections. The reaction conditions are first optimized at the light microscope level and then adapted for EM level observation. These methods allow for reliable detection of antigens at the cell surface, within the cell, and especially in membrane bounded organelles. Embedding and staining procedures are also optimized for sample visualization.
本精心撰写的单元描述了两种在电子显微镜水平上利用免疫过氧化物酶反应定位抗原的方法；一种用于贴壁培养细胞，另一种用于组织切片。反应条件首先在光学显微镜水平上进行优化，然后适用于电子显微镜水平的观察。这些方法能够可靠地检测细胞表面、细胞内，尤其是膜结合细胞器中的抗原。包埋和染色程序也针对样本可视化进行了优化。
