酵母蛋白的代谢标记与免疫沉淀
Metabolic labeling and immunoprecipitation of yeast proteins.
作者信息
Graham T R
机构信息
Vanderbilt University, Nashville, Tennessee, USA.
出版信息
Curr Protoc Cell Biol. 2001 May;Chapter 7:Unit 7.6. doi: 10.1002/0471143030.cb0706s06.
PMID:18228384
Abstract
The proteins of Saccharomyces cervsiae can be metabolically labeled, as described here, with (35)methionine and (35)cysteine or a hydrolysate of E. coli labeled with (35)O4(2-). After the labeling, protocols are provided for the mechanical disruption of the yeast cells or conversion to spheroplasts, with subsequent lysis before immunoprecipitation of the proteins.
摘要
酿酒酵母的蛋白质可以按照此处所述，用（35）甲硫氨酸和（35）半胱氨酸或用（35）O4（2-）标记的大肠杆菌水解产物进行代谢标记。标记后，提供了用于机械破碎酵母细胞或转化为原生质球的方案，随后在蛋白质免疫沉淀之前进行裂解。
Zotero 插件