从生物合成标记细胞中对分泌囊泡蛋白进行顺序免疫沉淀

Sequential Immunoprecipitation of Secretory Vesicle Proteins from Biosynthetically Labelled Cells.

作者信息

Guest Paul C

机构信息

Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255 F/01, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, Brazil.

出版信息

Adv Exp Med Biol. 2017;974:157-165. doi: 10.1007/978-3-319-52479-5_11.

DOI:10.1007/978-3-319-52479-5_11

PMID:28353232

Abstract

Pulse radiolabelling of cells with radioactive amino acids is a common method for studying the biosynthesis of proteins. The labelled proteins can then be immunoprecipitated and analysed by electrophoresis and imaging techniques. This chapter presents a protocol for the biosynthetic labelling and immunoprecipitation of pancreatic islet proteins which are known to be affected in psychiatric disorders such as schizophrenia.

摘要

用放射性氨基酸对细胞进行脉冲放射性标记是研究蛋白质生物合成的常用方法。然后可以对标记的蛋白质进行免疫沉淀，并通过电泳和成像技术进行分析。本章介绍了一种用于胰腺胰岛蛋白生物合成标记和免疫沉淀的方案，已知这些蛋白在精神分裂症等精神疾病中会受到影响。