Zhou Benhong, Wu Zhenhua, Li Xiaojun, Zhang Jie, Hu Xianming
Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan City, Hubei Province, People's Republic of China.
Phytochem Anal. 2008 Jan-Feb;19(1):86-9. doi: 10.1002/pca.1054.
Two novel methods for the analysis of ellagic acid in pomegranate (Punica granatum) rinds are proposed. Capillary electrophoresis (CE) was performed in a bare fused-silica capillary using a buffer solution of tri(hydroxymethyl)aminomethane:potassium dihydrogen phosphate (pH 8.4) with an applied voltage of 20 kV and UV detection at 254 nm. HPLC analysis was performed with a Zobax SB C(18) column and a mobile phase consisting of methanol:ethyl acetate:potassium dihydrogen phosphate: phosphoric acid at a flow rate of 1.0 mL/min. Under optimised conditions, the HPLC retention and the CE migration times for ellagic acid were 10.32 and 12.23 min, respectively. Calibration curves of peak area vs. concentration gave correlation coefficients of 0.9999 for HPLC and 0.9990 for CE. The detection limits for HPLC and CE were 2.8 and 2.2 microg/mL, respectively. Average recoveries were 98.32 +/- 1.2% for HPLC and 96.52 +/- 2.8% for CE. Both methods were shown to be suitable for the determination of ellagic acid in pomegranate rinds extraction; however, the CE method required less solvent and gave better column efficiency, whilst the HPLC provided superior precision.
提出了两种分析石榴皮中鞣花酸的新方法。采用三（羟甲基）氨基甲烷：磷酸二氢钾（pH 8.4）缓冲溶液，在裸露的熔融石英毛细管中进行毛细管电泳（CE），施加电压20 kV，在254 nm处进行紫外检测。使用Zobax SB C(18)柱和由甲醇：乙酸乙酯：磷酸二氢钾：磷酸组成的流动相，流速为1.0 mL/min进行高效液相色谱（HPLC）分析。在优化条件下，鞣花酸的HPLC保留时间和CE迁移时间分别为10.32和12.23分钟。峰面积与浓度的校准曲线，HPLC的相关系数为0.9999，CE的相关系数为0.9990。HPLC和CE的检测限分别为2.8和2.2 μg/mL。HPLC的平均回收率为98.32±1.2%，CE的平均回收率为96.52±2.8%。两种方法均适用于石榴皮提取物中鞣花酸的测定；然而，CE方法所需溶剂较少，柱效更高，而HPLC的精密度更高。
