Anouti Suzanne, Vandenabeele-Trambouze Odile, Koval Dusan, Cottet Hervé
Institut des Biomolécules Max Mousseron (UMR 5247 CNRS-Université de Montpellier 1-Université de Montpellier 2), place Eugène Bataillon CC 017, 34095 Montpellier Cedex 5, France.
Anal Chem. 2008 Mar 1;80(5):1730-6. doi: 10.1021/ac702117h. Epub 2008 Jan 30.
Heart-cutting two-dimensional (2D) capillary electrophoresis (CE) in a single capillary was used for analysis of derivatized amino acids. A mixture of 12 amino acids derivatized with UV-active benzyl 4-(3-(2-chloroethyl)-3-nitrosoureido)butylcarbamate label served as a model of a moderately complex sample due to the presence of numerous derivatization byproducts. The first step of the heart-cutting 2D approach was sample cleanup by capillary zone electrophoresis (CZE) in borate electrolyte. Then, only a selected portion of the first-dimension separation was transferred into the second dimension of the separation by a specific voltage and pressure program. Finally, the zone of derivatized amino acids was separated by micellar electrokinetic chromatography in a borate-sodium dodecyl sulfate system. The whole 2D process can be performed in a conventional CE analyzer without any interface for connection of the two separation modes. Intraday repeatability of the total migration time was 2%. In general, the heart-cutting 2D-CE methodology in a single capillary can be adapted for any CE mode regardless of the direction and velocity of electroosmotic flow and position of the fraction of interest in the first dimension (i.e., first, last, or intermediate fraction).
采用单毛细管的心切二维（2D）毛细管电泳（CE）对衍生化氨基酸进行分析。由于存在大量衍生化副产物，用具有紫外活性的苄基4-(3-(2-氯乙基)-3-亚硝基脲基)丁基氨基甲酸酯标记衍生化的12种氨基酸混合物用作中等复杂样品的模型。心切二维方法的第一步是在硼酸盐电解质中通过毛细管区带电泳（CZE）进行样品净化。然后，通过特定的电压和压力程序，仅将第一维分离的选定部分转移到分离的第二维中。最后，在硼酸盐-十二烷基硫酸钠系统中通过胶束电动色谱法分离衍生化氨基酸区域。整个二维过程可以在传统的CE分析仪中进行，无需任何连接两种分离模式的接口。总迁移时间的日内重复性为2%。一般来说，单毛细管的心切二维CE方法可适用于任何CE模式，而与电渗流的方向和速度以及感兴趣部分在第一维中的位置（即第一、最后或中间部分）无关。
