Cai X, Li C, Du G, Cao Z
Department of Periodontology, School and Hospital of Stomatology, Key Laboratory for Oral Biomedical Engineering of Ministry of Education, Wuhan University, Wuhan, China.
J Periodontal Res. 2008 Feb;43(1):14-21. doi: 10.1111/j.1600-0765.2007.00989.x.
Baicalin is a flavonoid compound purified from the medicinal plant, Scutellaria baicalensis Georgi, and has been reported to possess anti-inflammatory and antioxidant activities. The purpose of this study was to test the ability of baicalin to influence the progression of experimental periodontitis in rats, as well as the expression of cyclooxygenase-2 and inducible nitric oxide synthase.
Adult male Sprague-Dawley rats were subjected to placement of a nylon thread around the bilateral lower first molars and killed after 7 d. Baicalin (50, 100 or 200 mg/kg) was supplied to the animals by oral gavage, starting 1 d before the induction of periodontitis. The ligature group consisted of rats subjected to periodontitis and receiving vehicle (0.5% carboxymethylcellulose) alone. The alveolar bone loss and the area fraction occupied by collagen fibers were assessed. The expression of cyclooxygenase-2 and inducible nitric oxide synthase protein in the gingiva were detected by immunohistochemistry and western blotting.
Baicalin-treated groups presented with lower alveolar bone loss than that of the ligature group, reaching statistical significance at the dose of 200 mg/kg (p = 0.009). The area fraction of collagen fibers was significantly higher in the baicalin (200 mg/kg)-treated group than in the ligature group (p = 0.047). Baicalin treatment significantly down-regulated the protein expression for cyclooxygenase-2 (p = 0.000) and inducible nitric oxide synthase (p = 0.003), compared with the ligature group.
Baicalin protects against tissue damage in ligature-induced periodontitis in rats, which might be mediated, in part, by its inhibitory effect on the expression of cyclooxygenase-2 and inducible nitric oxide synthase. These activities could support the continued investigation of baicalin as a potential therapeutic agent in periodontal disease.
黄芩苷是从药用植物黄芩中提纯的一种黄酮类化合物，据报道具有抗炎和抗氧化活性。本研究旨在测试黄芩苷对大鼠实验性牙周炎进展的影响，以及对环氧合酶 -2 和诱导型一氧化氮合酶表达的影响。
成年雄性Sprague-Dawley大鼠双侧下颌第一磨牙周围放置尼龙线，7天后处死。在诱导牙周炎前1天开始通过灌胃给予大鼠黄芩苷（50、100或200mg/kg）。结扎组由患牙周炎且仅接受赋形剂（0.5%羧甲基纤维素）的大鼠组成。评估牙槽骨吸收和胶原纤维所占面积分数。通过免疫组织化学和蛋白质印迹法检测牙龈中环氧合酶-2和诱导型一氧化氮合酶蛋白的表达。
黄芩苷治疗组的牙槽骨吸收低于结扎组，在200mg/kg剂量时达到统计学显著性（p = 0.009）。黄芩苷（200mg/kg）治疗组的胶原纤维面积分数显著高于结扎组（p = 0.047）。与结扎组相比，黄芩苷治疗显著下调了环氧合酶-2（p = 0.000）和诱导型一氧化氮合酶（p = 0.003）的蛋白表达。
黄芩苷可保护大鼠结扎诱导的牙周炎中的组织损伤，这可能部分是由其对环氧合酶-2和诱导型一氧化氮合酶表达的抑制作用介导的。这些活性支持将黄芩苷作为牙周疾病潜在治疗剂的持续研究。
