Department of Surgery, Division of Orthopaedics, Bahia School of Medicine and Public Health, 275 D, João VI Ave, Salvador-Bahia, Brazil.
J Orthop Surg Res. 2008 Jan 29;3:4. doi: 10.1186/1749-799X-3-4.
Animal models of fracture consolidation are fundamental for the understanding of the biological process of bone repair in humans, but histological studies are rare and provide only qualitative results. The objective of this article is to present the histomorphometric study of the bone healing process using an experimental model of osteotomy in rabbit fibula without interference of synthesis material.
Fifteen rabbits were submitted to fibular osteotomy without any fixation device. Groups of five animals were submitted to pharmacological euthanasia during a period of one (group A), two (group B) and four weeks (group C) after osteotomy. Histomorphometric evaluation was performed in the histological sections.
During week one there was intense cellularity (67/field), a large amount of woven bone (75.7%) and a small amount of lamellar bone (7.65%). At two weeks there was a decrease in woven bone (41.59%) and an increase in lamellar bone (15.16%). At four weeks there was a decrease of cellularity (19.17/field) and lamellar bone (55.56%) exceeded the quantity of woven bone (31.68%).
Histomorphometric (quantitative) evaluation of the present study was shown to be compatible with bone healing achieved in qualitative experimental models that have been commended in the literature.
骨折愈合的动物模型对于理解人类骨骼修复的生物学过程至关重要，但组织学研究很少，并且仅提供定性结果。本文的目的是介绍一种实验性兔腓骨切开术模型，在不干扰合成材料的情况下，对骨愈合过程进行组织形态计量学研究。
15 只兔子接受了无任何固定装置的腓骨切开术。5 只动物一组，在切开后 1 周（A 组）、2 周（B 组）和 4 周（C 组）时进行药理学安乐死。在组织学切片中进行组织形态计量学评估。
第 1 周细胞活性强烈（67/视野），编织骨大量（75.7%），板层骨少量（7.65%）。第 2 周编织骨减少（41.59%），板层骨增加（15.16%）。第 4 周细胞活性（19.17/视野）下降，板层骨（55.56%）超过编织骨（31.68%）的数量。
本研究的组织形态计量学（定量）评估与文献中备受推崇的定性实验模型中观察到的骨愈合情况相符。
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