Ferriola P C, Nettesheim P
Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Science Research, Triangle Park, NC 27709.
J Radiat Res. 1991 Dec;32 Suppl 2:277-87. doi: 10.1269/jrr.32.supplement2_277.
The role of peptide growth factors in the process of multistage carcinogenesis of rat tracheal epithelial (RTE) cells was assessed by examining growth factor requirements and expression of growth factors and their receptors in normal and transformed RTE cells. Transformed RTE cell lines show decreased requirements for bovine pituitary extract, insulin and epidermal growth factor compared to primary RTE cells in culture. An autocrine role for TGF alpha in transformed RTE cells is suggested by data showing TGF alpha production and decreased proliferation in the presence of TGF alpha antisera and TGF alpha/EGF receptor kinase inhibitor. Therefore, decreased EGF requirements in transformed RTE cells could be explained by autocrine TGF alpha regulation. In contrast, no evidence for an insulin/IGF-I autocrine pathway could be detected in transformed RTE cells. These data indicate that multiple alterations in growth factor pathways occur in transformed RTE cells.
通过检测正常和转化的大鼠气管上皮（RTE）细胞中生长因子需求以及生长因子及其受体的表达，评估了肽生长因子在大鼠气管上皮细胞多阶段癌变过程中的作用。与培养中的原代RTE细胞相比，转化的RTE细胞系对牛垂体提取物、胰岛素和表皮生长因子的需求降低。数据显示转化的RTE细胞中存在TGFα产生，并且在存在TGFα抗血清和TGFα/EGF受体激酶抑制剂的情况下细胞增殖减少，这表明TGFα在转化的RTE细胞中具有自分泌作用。因此，转化的RTE细胞中对EGF需求的降低可以通过自分泌TGFα调节来解释。相比之下，在转化的RTE细胞中未检测到胰岛素/IGF-I自分泌途径的证据。这些数据表明，转化的RTE细胞中生长因子途径发生了多种改变。
