von Gersdorff Jørgensen L, Heinecke R D, Kania P, Buchmann K
Department of Veterinary Pathobiology, Laboratory of Fish Diseases, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C., Denmark.
J Fish Dis. 2008 Feb;31(2):127-34. doi: 10.1111/j.1365-2761.2007.00875.x.
Three hundred and one parr of wild salmon, Salmo salar L., from three river systems in Denmark were examined in December 2005 and August 2006 for gyrodactylid infections. The species determination was done by morphological analyses of the opisthaptoral hard parts and molecular analyses of the internal transcribed spacer (ITS) region using PCR restriction fragment length polymorphism (RFLP) and sequencing. Five species of the genus Gyrodactylus were found: Gyrodactylus derjavini, Gyrodactylus teuchis, Gyrodactylus truttae, Gyrodactylus arcuatus and Gyrodactylus salaris. The parasite species G. derjavini dominated over G. teuchis and G. truttae and only one specimen of G. arcuatus and one specimen of G. salaris were recovered. This specimen of G. salaris had the same mutation in the ITS-region as a previously described non-pathogenic Danish G. salaris variant. The prevalence of gyrodactylids on the salmon ranged from 0% to 100% but the mean abundance never exceeded 12.8.
2005年12月和2006年8月,对来自丹麦三个河流系统的301尾野生鲑鱼(Salmo salar L.)进行了三代虫感染情况检查。通过对后吸器硬体部分进行形态学分析,并利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和测序技术对内部转录间隔区(ITS)进行分子分析来确定物种。共发现了五种三代虫属物种:德氏三代虫(Gyrodactylus derjavini)、特氏三代虫(Gyrodactylus teuchis)、鲑三代虫(Gyrodactylus truttae)、弓形三代虫(Gyrodactylus arcuatus)和鲑居三代虫(Gyrodactylus salaris)。寄生虫物种德氏三代虫的数量超过了特氏三代虫和鲑三代虫,仅发现了1尾弓形三代虫和1尾鲑居三代虫。这尾鲑居三代虫在ITS区域的突变与先前描述的丹麦非致病性鲑居三代虫变种相同。鲑鱼身上三代虫的感染率在0%至100%之间,但平均丰度从未超过12.8。