Miyayama H, Doellgast G J, Memoli V, Gandbhir L, Fishman W H
Cancer. 1976 Sep;38(3):1237-46. doi: 10.1002/1097-0142(197609)38:3<1237::aid-cncr2820380325>3.0.co;2-2.
Immunoperoxidase staining for Regan isoenzyme of alkaline phosphatase was performed on cryostat sections of five human tumor tisssues. With a direct immunoperoxidase staining for the localization of Regan isoenzyme at the light and electron microscope levels, sections previously fixed with 0.05 M phosphate-buffered 4% paraformaldehyde were reacted with rabbit antisera to human placenta alkaline phosphatase conjugated to horseradish peroxidase. Comparison of conventional histochemistry and immunohistochemistry for Regan isoenzyme indicated that strong specific immunoperoxidase staining appeared on the cell membrane surface, and a diffuse one, in the cytoplasm of lung and colon cancer tissue cells showing L-phenylalanine-sensitive alkaline phosphatase. No immunoperoxidase reaction was obtained in tumor cells showing sensitivity to L-homoarginine or lacking aklaline phosphatase activity.
对五例人类肿瘤组织的低温切片进行了碱性磷酸酶雷根同工酶的免疫过氧化物酶染色。采用直接免疫过氧化物酶染色法在光镜和电镜水平定位雷根同工酶，将先前用0.05M磷酸盐缓冲的4%多聚甲醛固定的切片与辣根过氧化物酶偶联的人胎盘碱性磷酸酶兔抗血清反应。雷根同工酶的传统组织化学与免疫组织化学比较表明，在显示L-苯丙氨酸敏感碱性磷酸酶的肺癌和结肠癌组织细胞的细胞膜表面出现强特异性免疫过氧化物酶染色，在细胞质中出现弥漫性染色。在对L-高精氨酸敏感或缺乏碱性磷酸酶活性的肿瘤细胞中未获得免疫过氧化物酶反应。
