A study on the technology of mass cell culture of American ginseng (Panax quinquefolium L.).

The culture broth volume fitted for cell suspension culture of plants was 1/5 to 2/5 of shake flask capacity. The dissolved oxygen (DO) output of the detector increased with the increase of the temperature. The viscosity of American ginseng (Panax quinquefolium L.) cell cultures was obviously higher than that of the fresh medium. An appropriate agitation speed was 60 rpm for fermentation culture of American ginseng cells. When we increased osmotic pressure stress by the addition of mannitol to the nutrient medium in fermentation culture, the content of saponin of the cell cultures was increased greatly but the cell growth was inhibited. The study provides data for designing an appropriate fermentor to culture American ginseng cells on a large scale.