Calvo Jennifer A, Daniels Thomas G, Wang Xiaomei, Paul Angelika, Lin Jiandie, Spiegelman Bruce M, Stevenson Susan C, Rangwala Shamina M
Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, USA.
J Appl Physiol (1985). 2008 May;104(5):1304-12. doi: 10.1152/japplphysiol.01231.2007. Epub 2008 Jan 31.
The induction of peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha), a key regulator of mitochondriogenesis, is well-established under multiple physical exercise regimens, including, endurance, resistance, and sprint training. We wanted to determine if increased expression of PGC-1alpha in muscle is sufficient to improve performance during exercise in vivo. We demonstrate that muscle-specific expression of PGC-1alpha improves the performance during voluntary as well as forced exercise challenges. Additionally, PGC-1alpha transgenic mice exhibit an enhanced performance during a peak oxygen uptake exercise test, demonstrating an increased peak oxidative capacity, or whole body oxygen uptake. This increased ability to perform in multiple exercise paradigms is supported by enhanced mitochondrial function as suggested by increased mitochondrial gene expression, mitochondrial DNA, and mitochondrial enzyme activity. Thus this study demonstrates that upregulation of PGC-1alpha in muscle in vivo is sufficient to greatly improve exercise performance under various exercise paradigms as well as increase peak oxygen uptake.
过氧化物酶体增殖物激活受体γ共激活因子-1α（PGC-1α）是线粒体生成的关键调节因子，在多种体育锻炼方案（包括耐力训练、阻力训练和短跑训练）下，其诱导作用已得到充分证实。我们想确定肌肉中PGC-1α表达的增加是否足以改善体内运动期间的表现。我们证明，PGC-1α的肌肉特异性表达可改善自愿运动以及强迫运动挑战期间的表现。此外，PGC-1α转基因小鼠在峰值摄氧量运动测试中表现增强，表明峰值氧化能力或全身摄氧量增加。线粒体基因表达、线粒体DNA和线粒体酶活性增加表明线粒体功能增强，这支持了在多种运动模式下这种增强的运动能力。因此，本研究表明，体内肌肉中PGC-1α的上调足以在各种运动模式下极大地改善运动表现，并增加峰值摄氧量。
