Bouzahzah Boumediene, Yurchenko Vyacheslav, Nagajyothi Fnu, Hulit James, Sadofsky Moshe, Braunstein Vicki L, Mukherjee Shankar, Weiss Hannah, Machado Fabiana S, Pestell Richard G, Lisanti Michael P, Tanowitz Herbert B, Albanese Chris
Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Cell Cycle. 2008 Feb 15;7(4):500-3. doi: 10.4161/cc.7.4.5327. Epub 2007 Nov 18.
Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L(6)E(9) myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.
感染寄生虫克氏锥虫会引发恰加斯病。在本研究中，我们证明，受克氏锥虫（图拉亨株）感染的成肌细胞中细胞周期蛋白D1的表达有所增加。为研究细胞周期蛋白D1表达增加的可能机制，我们用细胞周期蛋白D1荧光素酶报告基因构建体转染L(6)E(9)成肌细胞，并使其感染克氏锥虫。没有证据表明启动子活性增加。此外，定量PCR并未显示感染期间细胞周期蛋白D1信使有任何变化。而且，我们证明感染后细胞周期蛋白D1蛋白显著稳定。总体而言，这些数据表明，克氏锥虫感染会在翻译后增加细胞周期蛋白D1的蛋白丰度。
