Zhou L G, Zheng G Z, Gan F Y, Wang S L, Yang C R, Xu C
Kunming Institute of Botany, Academia Sinica.
Yao Xue Xue Bao. 1991;26(11):876-80.
Five main saponins were separated from the cell cultures of Panax notoginseng (Burk) F. H. Chen. They were sanchinoside Rb1, Re, R1, Rg1 and Rh1 identified by TLC, HPLC, IR, M. P., 13CNMR and EI-MS. Saponin components of the cell cultures were almost the same as those of the cultivated plants. But the content of saponins was different between the cell cultures and the cultivated plants. Saponin extraction and separation procedure suitable for the cell cultures has to be different from that for the cultivated plants.
从三七(Burk)F.H. Chen的细胞培养物中分离出了五种主要皂苷。通过薄层色谱法(TLC)、高效液相色谱法(HPLC)、红外光谱法(IR)、熔点(M.P.)、碳-13核磁共振波谱法(13CNMR)和电子轰击质谱法(EI-MS)鉴定,它们分别是三七皂苷Rb1、Re、R1、Rg1和Rh1。细胞培养物中的皂苷成分与栽培植物的几乎相同。但是细胞培养物和栽培植物中的皂苷含量有所不同。适用于细胞培养物的皂苷提取和分离程序必须与适用于栽培植物的不同。