Li M, Wang J F, Han J S, Zhang J T
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Yao Xue Xue Bao. 1991;26(12):890-4.
The intracellular free Ca2+ concentration was measured in freshly dissociated brain cells prepared from neonatal rats using the fluorescent Ca2+ indicator Fura-2/AM. Cytosolic Ca2+ concentration of resting cells was calculated to be 240 +/- 5 nmol/L. Depolarization with high K+ resulted in an over 100% increase in intracellular Ca2+ concentration, and this increase could be prevented or reversed by verapamil or nifedipine known to block voltage-sensitive Ca channels. These results suggest that the adoption of Fura-2/AM method in freshly dissociated rat brain cells is a useful and relatively easily applicable technique for monitoring intracellular Ca2+ changes.
使用荧光钙指示剂Fura-2/AM测量新生大鼠新鲜分离的脑细胞中的细胞内游离钙离子浓度。静息细胞的胞质钙离子浓度经计算为240±5 nmol/L。高钾去极化导致细胞内钙离子浓度增加超过100%，而这种增加可被已知能阻断电压敏感性钙通道的维拉帕米或硝苯地平阻止或逆转。这些结果表明，在新鲜分离的大鼠脑细胞中采用Fura-2/AM方法是一种用于监测细胞内钙离子变化的有用且相对易于应用的技术。
