Wang Ying, Zhang Lu, Pan JingWei, Xie Kun, Li ShiQi, Wang ZhiRu, Lin LongNian
Key Laboratory of Brain Functional Genomics of Ministry of Education, East China Normal University, Shanghai 200062, China.
Sci China C Life Sci. 2008 Feb;51(2):120-6. doi: 10.1007/s11427-008-0013-0.
By simultaneously recording the activity of individual neurons and field potentials in freely behaving mice, we found two types of interneurons firing at high frequency in the hippocampal CA1 region, which had high correlations with characteristic sharp wave-associated ripple oscillations (100-250 Hz) during slow-wave sleep. The firing of these two types of interneurons highly synchronized with ripple oscillations during slow-wave sleep, with strongly increased firing rates corresponding to individual ripple episodes. Interneuron type I had at most one spike in each sub-ripple cycle of ripple episodes and the peak firing rate was 310+/-33.17 Hz. Interneuron type II had one or two spikes in each sub-ripple cycle and the peak firing rate was 410+/-47.61 Hz. During active exploration, their firing was phase locked to theta oscillations with the highest probability at the trough of theta wave. Both two types of interneurons increased transiently their firing rates responding to the startling shake stimuli. The results showed that these two types of high-frequency interneurons in the hippocampal CA1 region were involved in the modulation of the hippocampal neural network during different states.
通过同时记录自由活动小鼠单个神经元的活动和场电位，我们在海马CA1区发现了两种高频放电的中间神经元，它们在慢波睡眠期间与特征性的尖波相关涟漪振荡（100 - 250赫兹）高度相关。这两种中间神经元在慢波睡眠期间的放电与涟漪振荡高度同步，对应于单个涟漪事件时放电率显著增加。I型中间神经元在涟漪事件的每个子涟漪周期中最多有一个尖峰，峰值放电率为310±33.17赫兹。II型中间神经元在每个子涟漪周期中有一个或两个尖峰，峰值放电率为410±47.61赫兹。在主动探索期间，它们的放电与θ振荡相位锁定，在θ波谷时概率最高。两种中间神经元对惊吓震动刺激均短暂增加其放电率。结果表明，海马CA1区的这两种高频中间神经元参与了不同状态下海马神经网络的调制。
