Walton A P, Wei G T, Liang Z, Michel R G, Morris J B
Department of Chemistry, University of Connecticut, Storrs 06269-3060.
Anal Chem. 1991 Feb 1;63(3):232-40. doi: 10.1021/ac00003a009.
The coupling of a high-performance liquid chromatograph with a sensitive and selective laser-excited atomic fluorescence spectrometry (LEAFS) detector is described. In connection with this, a study of the signal and noise characteristics of instrumentation for dispersive, nondispersive, and front surface LEAFS is reported together with a comparison of the sensitivity and selectivity achieved with high-performance liquid chromatography (HPLC)-flame LEAFS, HPLC-ultraviolet (UV), and HPLC-continuum source excited flame atomic fluorescence spectrometry (AFC) instrumentation. The HPLC-flame LEAFS instrumentation was applied to an investigation of the Mn species responsible for (methylcyclopentadienyl)manganese tricarbonyl (MMT) toxicity in rats. The detection limits for various organomanganese species by HPLC-flame LEAFS ranged from 8 to 22 pg of manganese. Recovery of these compounds from rat urine varied between 80% and 100%, with a reproducibility of between 4% and 8% relative standard deviation. Preliminary data for the HPLC-flame LEAFS determination of toxic alkyltin compounds are reported.
本文描述了高效液相色谱仪与灵敏且具有选择性的激光激发原子荧光光谱法（LEAFS）检测器的联用。与此相关，本文报道了对色散型、非色散型和前表面LEAFS仪器的信号与噪声特性的研究，并比较了高效液相色谱（HPLC）-火焰LEAFS、HPLC-紫外（UV）以及HPLC-连续光源激发火焰原子荧光光谱法（AFC）仪器所实现的灵敏度和选择性。HPLC-火焰LEAFS仪器被用于研究大鼠体内对（甲基环戊二烯基）三羰基锰（MMT）毒性负责的锰物种。HPLC-火焰LEAFS对各种有机锰物种的检测限为8至22皮克锰。这些化合物从大鼠尿液中的回收率在80%至100%之间，相对标准偏差的重现性在4%至8%之间。本文还报道了HPLC-火焰LEAFS测定有毒烷基锡化合物的初步数据。
