Briere Catherine A, Hosgood Giselle, Morgan Timothy W, Hedlund Cheryl S, Hicks Merrin, McConnico Rebecca S
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Am J Vet Res. 2008 Feb;69(2):174-81. doi: 10.2460/ajvr.69.2.174.
To measure effects of carprofen on conductance and permeability to mannitol and histologic appearance in canine colonic mucosa.
Colonic mucosa from 13 mature mixed-breed dogs. Procedures-Sections of mucosa from the transverse colon and proximal and distal portions of the descending colon were obtained immediately after dogs were euthanized. Sections were mounted in Ussing chambers. Carprofen (400 microg/mL) was added to the bathing solution for treated sections. Conductance was calculated at 15-minute intervals for 240 minutes. Flux of mannitol was calculated for three 1-hour periods. Histologic examination of sections was performed after experiments concluded. Conductance was graphed against time for each chamber, and area under each curve was calculated. Conductance X time, flux of mannitol, and frequency distribution of histologic findings were analyzed for an effect of region and carprofen.
Carprofen significantly increased mean conductance X time, compared with values for control (untreated) sections for all regions of colon. Carprofen significantly increased mean flux of mannitol from period 1 to period 2 and from period 2 to period 3 for all regions of colon. Carprofen caused a significant proportion of sections to have severe sloughing of cells and erosions involving >or= 10% of the epithelium, compared with control sections.
Carprofen increased in vitro conductance and permeability to mannitol in canine colonic mucosa. Carprofen resulted in sloughing of cells and erosion of the colonic mucosa. These findings suggested that carprofen can compromise the integrity and barrier function of the colonic mucosa of dogs.
测定卡洛芬对犬结肠黏膜电导率、对甘露醇的通透性及组织学外观的影响。
13只成年杂种犬的结肠黏膜。
犬实施安乐死后，立即获取横结肠以及降结肠近端和远端的黏膜切片。将切片安装于尤斯灌流小室中。向处理组切片的浴液中加入卡洛芬（400微克/毫升）。每隔15分钟计算一次电导率，共计算240分钟。计算三个1小时时间段内甘露醇的通量。实验结束后对切片进行组织学检查。针对每个小室，绘制电导率随时间变化的曲线，并计算每条曲线下的面积。分析电导率×时间、甘露醇通量以及组织学检查结果的频率分布，以研究部位和卡洛芬的影响。
与结肠所有区域的对照（未处理）切片相比，卡洛芬显著增加了平均电导率×时间。在结肠所有区域，卡洛芬使甘露醇的平均通量从第1阶段到第2阶段以及从第2阶段到第3阶段均显著增加。与对照切片相比，卡洛芬导致相当比例的切片出现严重的细胞脱落和累及≥10%上皮的糜烂。
卡洛芬增加了犬结肠黏膜体外的电导率及对甘露醇的通透性。卡洛芬导致细胞脱落和结肠黏膜糜烂。这些发现表明卡洛芬会损害犬结肠黏膜的完整性和屏障功能。
