卵巢癌中人类HtrA1、HtrA2和HtrA3的mRNA及蛋白质水平变化

Changes in mRNA and protein levels of human HtrA1, HtrA2 and HtrA3 in ovarian cancer.

作者信息

Narkiewicz Joanna, Klasa-Mazurkiewicz Dagmara, Zurawa-Janicka Dorota, Skorko-Glonek Joanna, Emerich Janusz, Lipinska Barbara

机构信息

Department of Biochemistry, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland.

出版信息

Clin Biochem. 2008 May;41(7-8):561-9. doi: 10.1016/j.clinbiochem.2008.01.004. Epub 2008 Jan 16.

DOI:10.1016/j.clinbiochem.2008.01.004

PMID:18241672

Abstract

OBJECTIVES

Expression of human HtrA1, HtrA2, HtrA3 and TGF-beta1 genes was examined in ovarian tissue specimens including 19 normal ovaries, 20 benign tumors, 7 borderline tumors, 44 cancers and 8 Krukenberg tumors.

DESIGN AND METHODS

mRNA and protein levels were evaluated by semi-quantitative RT-PCR and Western-blotting methods, respectively.

RESULTS

A statistically significant decrease of HtrA1 and HtrA3 expression in ovarian tumors comparing to normal tissues was observed. A dramatic decrease of HtrA3 mRNA and protein levels in all tumor tissue groups, and a loss of HtrA3 protein in 30% malignant tumors were found. A significant decrease of HtrA1 mRNA, and of HtrA3 mRNA and protein in malignant tumors compared to benign tumors was revealed. HtrA2 expression in tumor tissues was slightly decreased. Expression of TGF-beta1 in tumor tissues was not significantly different compared to control tissues.

CONCLUSIONS

Our results show downregulation of HtrA1 and HtrA3 genes' expression in different types of ovarian tumors and give additional evidence that these genes may function as tumor suppressors.

摘要

目的

检测人HtrA1、HtrA2、HtrA3和转化生长因子β1（TGF-β1）基因在包括19例正常卵巢、20例良性肿瘤、7例交界性肿瘤、44例癌和8例库肯勃瘤的卵巢组织标本中的表达。

设计与方法

分别采用半定量逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法评估mRNA和蛋白质水平。

结果

与正常组织相比，观察到卵巢肿瘤中HtrA1和HtrA3表达有统计学意义的降低。在所有肿瘤组织组中发现HtrA3 mRNA和蛋白质水平显著降低，且在30%的恶性肿瘤中HtrA3蛋白缺失。与良性肿瘤相比，恶性肿瘤中HtrA1 mRNA、HtrA3 mRNA和蛋白质显著降低。肿瘤组织中HtrA2表达略有降低。与对照组织相比，肿瘤组织中TGF-β1的表达无显著差异。