Saida N Ben, Thabet L, Messadi A, Bouselmi K, Turki A, Boukadida J
Microbiology and Immunology Laboratory UR 16/02, CHU Farhat-Hached, avenue Ibn-Jazzar, Sousse, Tunisia.
Burns. 2008 Sep;34(6):829-34. doi: 10.1016/j.burns.2007.09.008. Epub 2008 Feb 1.
In order to investigate an outbreak of multidrug-resistant Providencia stuartii that occurred in a burn unit, we analyzed by pulsed-field gel electrophoresis (PFGE) all isolates of P. stuartii collected during 4 months of 2005 from patients and from a tracheal aspirator. Seventeen clinical isolates of P. stuartii, extended-spectrum beta-lactamase (ESBL) producing, were collected from 17 patients. All these isolates were nosocomially acquired. Three other isolates were collected from the aspirator probe, the aspirator reservoir and from the aspirator tube. Three different antibiotypes were identified without correlation with the genotype. Two PFGE types were obtained (types A and B) with predominance of one (type A) that was observed for 15 isolates. P. stuartii isolates collected from different components of the aspirator (probe, reservoir and tube) yielded PFGE type A. This study suggests the bi-clonality of the outbreak and that transmission of epidemic P. stuartii isolates was through a common source. The aspirator probe, contaminated from aspirator that functioned as a reservoir of bacteria, seems to be the route of transmission of P. stuartii. Furthermore, this study shows the utility of PFGE in typing for the purpose of understanding the epidemiological behaviour of P. stuartii and as a basis for the development of rational control strategies.
为调查烧伤病房发生的多重耐药性斯氏普罗威登斯菌暴发，我们采用脉冲场凝胶电泳（PFGE）对2005年4个月期间从患者和气管吸引器采集的所有斯氏普罗威登斯菌分离株进行了分析。从17例患者中收集了17株产超广谱β-内酰胺酶（ESBL）的斯氏普罗威登斯菌临床分离株。所有这些分离株均为医院获得性。另外3株分离株分别从吸引器探头、吸引器储液罐和吸引器管中采集。鉴定出3种不同的抗菌型，与基因型无相关性。获得了2种PFGE型（A和B型），其中15株分离株以A型为主。从吸引器不同部件（探头、储液罐和管）采集的斯氏普罗威登斯菌分离株为PFGE A型。本研究提示该暴发具有双克隆性，且流行的斯氏普罗威登斯菌分离株通过共同来源传播。作为细菌储存库的吸引器污染了吸引器探头，似乎是斯氏普罗威登斯菌的传播途径。此外，本研究显示了PFGE在分型中的作用，有助于了解斯氏普罗威登斯菌的流行病学行为，并为制定合理的控制策略提供依据。
