CD90+癌症干细胞在人类肝癌中的意义。

Significance of CD90+ cancer stem cells in human liver cancer.

作者信息

Yang Zhen Fan, Ho David W, Ng Michael N, Lau Chi Keung, Yu Wan Ching, Ngai Patricia, Chu Patrick W K, Lam Chi Tat, Poon Ronnie T P, Fan Sheung Tat

机构信息

Center for Cancer Research and Department of Surgery, The University of Hong Kong, Pokfulam, Hong Kong, China.

出版信息

Cancer Cell. 2008 Feb;13(2):153-66. doi: 10.1016/j.ccr.2008.01.013.

DOI:10.1016/j.ccr.2008.01.013

PMID:18242515

Abstract

This study characterized cancer stem cells (CSCs) in hepatocellular carcinoma (HCC) cell lines, tumor specimens, and blood samples. The CD90+ cells, but not the CD90(-) cells, from HCC cell lines displayed tumorigenic capacity. All the tumor specimens and 91.6% of blood samples from liver cancer patients bore the CD45(-)CD90+ population, which could generate tumor nodules in immunodeficient mice. The CD90+CD44+ cells demonstrated a more aggressive phenotype than the CD90+CD44(-) counterpart and formed metastatic lesions in the lung of immunodeficient mice. CD44 blockade prevented the formation of local and metastatic tumor nodules by the CD90+ cells. Differential gene expression profiles were identified in the CD45(-)CD90+ and CD45(-)CD90(-) cells isolated from tissue and blood samples from liver cancer patients and controls.

摘要

本研究对肝癌（HCC）细胞系、肿瘤标本和血液样本中的癌症干细胞（CSCs）进行了特征分析。肝癌细胞系中的CD90+细胞而非CD90(-)细胞具有致瘤能力。所有肿瘤标本以及91.6%的肝癌患者血液样本中都存在CD45(-)CD90+群体，该群体可在免疫缺陷小鼠中形成肿瘤结节。CD90+CD44+细胞表现出比CD90+CD44(-)细胞更具侵袭性的表型，并在免疫缺陷小鼠的肺部形成转移灶。CD44阻断可阻止CD90+细胞形成局部和转移肿瘤结节。在从肝癌患者和对照的组织及血液样本中分离出的CD45(-)CD90+和CD45(-)CD90(-)细胞中鉴定出了差异基因表达谱。