Silva César R, Airoldi Claudio, Collins Kenneth E, Collins Carol H
Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, 13084-971 Campinas, São Paulo, Brazil.
J Chromatogr A. 2008 May 16;1191(1-2):90-8. doi: 10.1016/j.chroma.2008.01.017. Epub 2008 Jan 11.
To further extend our studies in the search for reversed phases with enhanced durability at high pH, zirconized silica has now been explored as an alternative support. The synthesis of the new stationary phases involves silanization of a zirconium-modified silica support with a C(18) trifunctional silane, followed by endcapping. The chromatographic properties of the C(18) phases based on zirconized silica are similar to their titanized silica counterparts. Accelerated high pH stability tests, using phosphate mobile phases and elevated temperature, have shown that the zirconized silica phases have promising advantages not only over similarly prepared non-metalized phases but also over titanized silica C(18) phases.
为了进一步拓展我们在寻找高pH值下具有更高耐久性的反相固定相方面的研究，现已探索将锆化硅胶作为一种替代载体。新型固定相的合成包括用C(18)三官能硅烷对锆改性硅胶载体进行硅烷化，然后进行封端。基于锆化硅胶的C(18)固定相的色谱性能与其钛化硅胶对应物相似。使用磷酸盐流动相并在高温下进行的加速高pH稳定性测试表明，锆化硅胶固定相不仅比类似制备的未金属化固定相具有显著优势，而且比钛化硅胶C(18)固定相也具有优势。
